Journal: Molecular Metabolism

Article Title: Baricitinib counteracts metaflammation, thus protecting against diet-induced metabolic abnormalities in mice

doi: 10.1016/j.molmet.2020.101009

Figure Lengend Snippet: Baricitinib attenuates the HD-induced JAK-STAT pathway. Western blotting analysis for phosphorylation of Tyr 1007/1008 JAK1/2 in the skeletal muscle (A) and kidneys (B) and normalized to total JAK1/2 and for phosphorylation of Tyr 690 on STAT2 in the skeletal muscle (C) and kidneys (D) and normalized to total STAT2. All of the data are expressed as mean ± SEM for n = 6 per group. ∗p< 0.05 vs ND and •p< 0.05 vs HD.

Article Snippet: The antibodies used were rabbit anti-Tyr 1007/1008 JAK2 (#3776), rabbit anti-total JAK2 (#3230), rabbit anti-Tyr 690 STAT2 (Bioss Antibodies, bs-3428R), rabbit anti-total STAT2 (#72604), rabbit p21 (#2947), rabbit anti-Ser 307 IRS-1 (#2381), mouse anti-total IRS-1 (#3194), rabbit anti-Ser 473 AKT (#4060), rabbit anti-total AKT (#9272), rabbit anti-Ser 9 GSK-3β (#9332) and rabbit anti-total GSK-3β (9315).

Techniques: Western Blot

Journal: Brain : a journal of neurology

Article Title: Tyrosine kinase 2 variant influences T lymphocyte polarization and multiple sclerosis susceptibility.

doi: 10.1093/brain/awr010

Figure Lengend Snippet: Figure 2 The rs34536443 polymorphism localizes in the PTK domain of TYK2 and affects the activation state of the protein. (A) Three-dimensional PTK domain model of the TYK2 protein. The activation loop is green, where Tyr1054 and Tyr1055 (pale green) are shown in stick form. The aFG helix is yellow and the TYK2P1104A amino acid modification is red. (B) Sequence alignment for a part of the kinase domain of human JAK family proteins and the kinase domain of SRC and EGFR. TYK2* represents the pseudo-kinase domain of TYK2. Alignment starts from position 1050 for TYK2 and 761 for TYK2* domains. HS, MM, MD and XL are for Homo sapiens, Mus musculus, Monodelphis domestica and Xenopus laevis, respectively. On the top of the alignment, b-strands are indicated as arrows, a-helices as cylinders, and loops as lines. Colours used are the same as in (A). Solid triangles below the JAK family protein alignment represent completely conserved positions and open triangles represent positions where all amino acids belong to the same structural class (Smith and Smith, 1992). Quantification of the P-TYK2/TYK2 ratio (C) and the P-STAT2/STAT2 ratio (D) by western blot. Proteins were extracted from expanded lymphocytes stimulated with IFNb (1000 U/ml). Total proteins and their phosphorylated forms were quantified on samples from TYK2GG (n = 10) and TYK2GC (n = 9), individual and normalized to b-actin expression. Each bar represents mean SEM. *P 5 0.05.

Article Snippet: Primary antibodies and quantitative real-time polymerase chain reaction Rabbit antibodies for JAK1, STAT1, STAT2, and their phosphorylated forms (Tyr1022 and Tyr1023 for phospho-JAK1, Tyr701 for phosphoSTAT1 and Tyr690 for phospho-STAT2) were purchased from Cell Signalling.

Techniques: Activation Assay, Sequencing, Western Blot, Expressing